Therapeutic effects and molecular mechanisms of quercetin in gynecological disorders.

Quercetin is a representative flavonoid that is widely present in fruits, herbs, and vegetables. It is also an important active core component in traditional Chinese medicines. As an important flavonoid, quercetin has various properties and exerts antioxidant, anti-inflammatory, and cardioprotective effects. The public interest in quercetin is increasing, and quercetin has been used to prevent or treat numerous of diseases, such as polycystic ovary syndrome (PCOS), cancer, autoimmune diseases and chronic cardiovascular diseases, in clinical experiments and animal studies due to its powerful antioxidant properties and minimal side effects. Quercetin exerts marked pharmacological effects on gynecological disorders; however, there have been no reviews about the potential health benefits of quercetin in the context of gynecological disorders, including PCOS, premature ovary failure (POF), endometriosis (EM), ovarian cancer (OC), cervical cancer (CC) and endometrial carcinoma (EC). Thus, this review aimed to summarize the biological effects of quercetin on gynecological disorders and its mechanisms.

A potential Mg-enriched biochar fertilizer: Excellent slow-release performance and release mechanism of nutrients.

A potential Mg-enriched biochar fertilizer (MBF) was successfully synthesized via pyrolysis of MgCl2-enriched corn straw and high-efficiency reclaiming of N- and P-containing nutrients from biogas effluent. Mathematical modeling and column leaching method demonstrated that the MBF exhibited excellent slow-release performances of total P and N with sustainable release rates. Leaching experiment indicated that the final accumulative release ratios of N and P from MBF were 7 times and 6 times lower than those of chemical fertilizer (CF), respectively. The mechanism study reveals that the P-release performance of MBF was not only controlled by the low solubility of MgP precipitates formed on the biochar surface, but also enhanced by the 'P-trap' effect of MgO through re-precipitation process of PO43-. Meanwhile, the N-release behavior of MBF was dominated by the multi-effects of biochar carrier, including the confinement effect and electrostatic attraction for NH4+, as well as the hydrogen bonds and pore-filling effect for N-containing organic matter. In addition, MBF significantly promoted the corn growth and enhanced the nutrient uptake efficiency of corn. These results suggested that MBF may therefore have promising potential in sustainable agriculture application with multiple environmental benefits.

Molecular subtyping for Escherichia coli O157: H7 isolated in Taiwan.

Enterohaemorrhagic Escherichia coli O157: H7 is an important pathogen these days. Outbreaks of its infection have been reported all over the world, in Australia, Canada, Japan, the United States, south Africa, and various countries in Europe. In the summer of 2001, the first clinical infection by E. coli O157: H7 was identified in Taiwan. In this study, the standard procedures for molecular subtyping were applied to several strains collected in Taiwan as well as from elsewhere. The two molecular subtyping methods we used are pulsed-field gel electrophoresis (PFGE) and amplified fragment length polymorphism (AFLP). The isolates from the U.S.A., Canada, Japan, and Taiwan each showed a unique molecular fingerprinting pattern. The environmental strains isolated in Taiwan showed closer relationships with each other, and their similarity was in the range of 75-85%. The first clinical strain isolated in Taiwan in 2001 was similar to the strains from North America but not closely related to the Taiwanese environmental strains. Our surveys showed that some local E. coli O157: H7 strains did exist in Taiwan, but there had been only one official case report of the infection by local E. coli O157: H7. The eating habits of the people and the geographic distribution of the pathogen are considered crucial risk factors in Taiwan. The establishment of a database of our own and joining the global network database are important tasks if we want to control such agricultural and food-borne pathogens, and reduce the number of victims and amount sufferings, as well as the economic losses due to the infection.